A series of animal experiments has been carried out to investigate the potential antigenicity of the FCS (Fetal Calf Serum) which is commonly used to enhance viability of preserved aortic allograft. Aortic allografts were processed using nutrient
media
without FCS(control group) or with 10% FCS(study group). After 14 days of 4℃ cold storage and cryopreservation, antigenic expression of allograft endothelial cells were studied using immunohistochemical study. To determine antigenicity, level of
anti-MHC class I antibody, anti-MHC class II antibody and anti-ICAM l antibody were measured. There were no statistically significant differences in all antigenic expression between control group and study group (p=0.524 in MHC class I
expression,
p=0.897 in MHC class II expression, p=0.1305 in ICAM I expression). With this result, antigenicity provoking effect of FCS could not be proven. Thus, FCS may not be eliminated from the nutrient media for preservation of aortic allograft due to
its
proven benefit of cell viability enhancement.
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